Blue Key

In, four Blue Keys are found on the third level of Crossbone Isle. They are used to open blue doors on that floor, each of which have a treasure chest sealed behind. Each of these keys can open any of the blue doors. The keys are each set on tiny isolated platforms. To get each key, the player must use Catch while facing the key on a platform two squares away.

In, the Blue Key is an item found in the Jupiter Lighthouse. It grants access to one of the two "towers" where the player must activate a statue figuring a bowman in order to open the top of the lighthouse. A Blue Key found at Crossbone Isle in the first game can also be transferred to the second game by using data transfer, but its description will now match that given to the other Blue Key acquired normally, presenting it as usable for a door in the Jupiter Lighthouse instead of Crossbone Isle. Since Isaac's party will only join bearing the key right after Jupiter Lighthouse is completed, it is not possible to use the data transfer function for sequence breaking.